Evidence of involvement of the receptor for advanced glycation end-products (RAGE) in the adhesion of Helicobacter pylori to gastric epithelial cells

Armando Rojas; Ileana González; Boris Rodríguez; Jacqueline Romero; Héctor Figueroa; Jorge Llanos; Erik Morales; Ramón Pérez-Castro

doi:10.1016/j.micinf.2011.04.005

Evidence of involvement of the receptor for advanced glycation end-products (RAGE) in the adhesion of Helicobacter pylori to gastric epithelial cells

Microbes Infect. 2011 Sep;13(10):818-23. doi: 10.1016/j.micinf.2011.04.005. Epub 2011 May 12.

Authors

Armando Rojas¹, Ileana González, Boris Rodríguez, Jacqueline Romero, Héctor Figueroa, Jorge Llanos, Erik Morales, Ramón Pérez-Castro

Affiliation

¹ Biomedical Research Labs, Medicine Faculty, Catholic University of Maule, 3605 San Miguel Ave., Talca, Chile. arojasr@ucm.cl

PMID: 21609778
DOI: 10.1016/j.micinf.2011.04.005

Abstract

The adherence of Helicobacter pylori to gastric epithelial cells is required for prolonged persistence in the stomach and for induction of injury. Here, we first reported a new role of the receptor for advanced glycation end-products (RAGE) on the adherence of H. pylori to gastric epithelial cells, assessed by different methods and binding to immobilized RAGE. RAGE-targeted knock-down in MKN74 cell line markedly reduced not only the adhesion of H. pylori, but also the levels of IL-8 transcripts and protein released in response to infection. These data suggest that RAGE may represent a new factor on the pathogenesis of H. pylori infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Adhesion*
Cell Line, Tumor
Epithelial Cells / microbiology*
Gene Expression Profiling
Gene Knockdown Techniques
Helicobacter pylori / pathogenicity*
Host-Pathogen Interactions*
Humans
Interleukin-8 / biosynthesis
Receptor for Advanced Glycation End Products
Receptors, Immunologic / metabolism*

Substances

Interleukin-8
Receptor for Advanced Glycation End Products
Receptors, Immunologic