Send to

Choose Destination
See comment in PubMed Commons below
Methods Enzymol. 2011;497:221-37. doi: 10.1016/B978-0-12-385075-1.00010-X.

Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells.

Author information

  • 1Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.


Perturbations from environmental, genetic, and pharmacological sources can generate heterogeneous biological responses, even in genetically identical cells. Although these differences have important consequences on cell physiology and survival, they are often subsumed in measurements that average over the population. Here, we describe in detail how variability in adenoviral-mediated gene expression provides an effective means to map dose responses of signaling pathways. Cell-cell variability is inherent in gene delivery methods used in cell biology, which makes this approach adaptable to many existing experimental systems. We also discuss strategies to quantify biologically relevant inputs and outputs.

Copyright © 2011 Elsevier Inc. All rights reserved.

[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk