Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Virol J. 2011 May 20;8:248. doi: 10.1186/1743-422X-8-248.

Inhibition of hepatitis C virus 3a genotype entry through Glanthus Nivalis Agglutinin.

Author information

  • 1Division of Molecular Medicine, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan. usmancemb@gmail.com

Abstract

BACKGROUND:

Hepatitis C Virus (HCV) has two envelop proteins E1 and E2 which is highly glycosylated and play an important role in cell entry. Inhibition of virus at entry step is an important target to find antiviral drugs against HCV. Glanthus Nivalis Agglutinin (GNA) is a mannose binding lectin which has tendency for specific recognition and reversible binding to the sugar moieties of a wide variety of glycoproteins of enveloped viruses.

RESULTS:

In the present study, HCV pseudoparticles (HCVpp) for genotype 3a were produced to investigate the ability of GNA to block the HCV entry. The results demonstrated that GNA inhibit the infectivity of HCVpp and HCV infected serum in a dose-dependent manner and resulted in 50% reduction of virus at 1 ± 2 μg concentration. Molecular docking of GNA and HCV glycoproteins (E1 and E2) showed that GNA inhibit HCV entry by binding N-linked glycans.

CONCLUSION:

These results demonstrated that targeting the HCV glycans is a new approach to develop antiviral drugs against HCV.

PMID:
21599979
[PubMed - indexed for MEDLINE]
PMCID:
PMC3117843
Free PMC Article

Images from this publication.See all images (4)Free text

Figure 1
Figure 2
Figure 3
Figure 4
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Write to the Help Desk