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    J Virol. 1990 Jun;64(6):3135-8.

    Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.

    Source

    Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.

    Abstract

    Flow cytometry was used to detect cells infected with retroviral vectors encoding both simian virus 40 large T antigen and G418 resistance after indirect immunofluorescence staining using a T-antigen-specific monoclonal antibody and a fluorescein-conjugated secondary antibody. Titers of viral stocks determined by flow cytometry were equivalent to those determined by quantitation of G418-resistant colonies.

    PMID:
    2159569
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC249510
    Free PMC Article

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