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Macromol Rapid Commun. 2010 Jan 4;31(1):71-4. doi: 10.1002/marc.200900497. Epub 2009 Nov 18.

Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles.

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  • 1Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.


Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435.

Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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