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Methods Mol Biol. 2011;722:51-60. doi: 10.1007/978-1-61779-040-9_4.

Next-generation sequencing and potential applications in fungal genomics.

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  • 1Purdue Genomics Core Facility, Purdue University, West Lafayette, IN, USA. pmiguel@purdue.edu

Abstract

Since the first fungal genome was sequenced in 1996, sequencing technologies have advanced dramatically. In recent years, it has become possible to cost-effectively generate vast amounts of DNA sequence data using a number of cell- and electrophoresis-free sequencing technologies, commonly known as "next" or "second" generation. In this chapter, we present a brief overview of next-generation sequencers that are commercially available now. Their potential applications in fungal genomics studies are discussed.

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