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Methods Mol Biol. 2011;722:51-60. doi: 10.1007/978-1-61779-040-9_4.

Next-generation sequencing and potential applications in fungal genomics.

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  • 1Purdue Genomics Core Facility, Purdue University, West Lafayette, IN, USA.


Since the first fungal genome was sequenced in 1996, sequencing technologies have advanced dramatically. In recent years, it has become possible to cost-effectively generate vast amounts of DNA sequence data using a number of cell- and electrophoresis-free sequencing technologies, commonly known as "next" or "second" generation. In this chapter, we present a brief overview of next-generation sequencers that are commercially available now. Their potential applications in fungal genomics studies are discussed.

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