Abstract
We cloned a gene, sugE, from the chromosome of Enterobacter cloacae ATCC 13047. Analysis of the susceptibilities of the sugE-containing strain (Escherichia coli KAM32/pSUGE28) and sugE-deficient E. cloacae (EcΔsugE) showed that SugE confers resistance to cetyltrimethylammonium bromide, cetylpyridinium chloride, tetraphenylphosphonium, benzalkonium chloride, ethidium bromide, and sodium dodecyl sulfate. We also investigated expression of sugE. We confirm here that SugE from E. cloacae is an SMR family transporter as determined by observing its energy-dependent drug efflux activity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Base Sequence
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Drug Resistance, Multiple, Bacterial / genetics
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Enterobacter cloacae / drug effects*
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Enterobacter cloacae / genetics
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Enterobacter cloacae / metabolism*
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Escherichia coli / genetics
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Gene Knockout Techniques
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Membrane Transport Proteins / biosynthesis
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism*
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Microbial Sensitivity Tests
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Deletion
Substances
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Anti-Bacterial Agents
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Escherichia coli Proteins
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Membrane Proteins
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Membrane Transport Proteins
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Molecular Chaperones
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sugE protein, E coli