Send to:

Choose Destination
See comment in PubMed Commons below
Zhonghua Zhong Liu Za Zhi. 2011 Jan;33(1):18-22.

[Promoting effect of Chlamydia pneumoniae infection on human laryngeal carcinoma HEp-2 cell adhesion and migration].

[Article in Chinese]

Author information

  • 1Department of Pathophysiology, Basic Medical College, Tianjin Medical University, Tianjin 300070, China.



To explore the effect of Chlamydia pneumoniae ( infection on human laryngeal carcinoma cell line HEp-2 cell adhesion and migration, to further clarify the role and mechanism of infection in tumor metastasis.


HEp-2 cells were infected with after the culture and propagation of The cytopathic effect was observed by microscopy. Morphological characteristics of inclusions in HEp-2 cells were examined by fluorescence microscopy and acridine orange staining. The ultrastructural changes of inclusions in the HEp-2 cells were examined by transmission electron microscopy (TEM). Cell adhesion assay was performed to investigate the effect of infection on the adhesion of HEp-2 cells to collagen I. Wound-healing assay and transwell assay were performed to explore the effect of infection on HEp-2 cell migration.


At 72 h post-infection, infected-HEp-2 cells were swollen and partially desquamated. Numerous vacuoles (inclusions) were observed and inclusions occupied almost the whole cytoplasm of the HEp-2 cells. Grape-like inclusions were observed in the HEp-2 cells stained with acridine orange under a fluorescence microscope at 72 h after infection. Under TEM, there were more mature pear-shaped elementary bodies, but less larger and round reticulate bodies in the HEp-2 cells infected with for 72 h. In the cell adhesion assay, the A value in infection group was 0.669 ± 0.011, significantly higher than that in the control group (0.558 ± 0.005) at 2 h after infection (P < 0.001). The cell adhesion ratio in the infection group was 119.89%. The migration distance of infected-HEp-2 cells in the wound-healing assay was significantly longer than that of control cells at 24 h after infection (P < 0.05). HEp-2 cells infected with for 12 h migrated more than the control cells in the transwell assay (23.40 ± 2.41 vs 10.40 ± 1.67) (P < 0.001).

CONCLUSIONS: infection can significantly promote HEp-2 cell adhesion to collagen I and migration of HEp-2 cells, indicating that infection may play an important role in promoting the metastasis of laryngeal cancer.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk