Nucleic Acids Res. 2011 Jul;39(Web Server issue):W551-6. Epub 2011 May 11.

BiQ Analyzer HT: locus-specific analysis of DNA methylation by high-throughput bisulfite sequencing.

Lutsik P, Feuerbach L, Arand J, Lengauer T, Walter J, Bock C.

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Department of Genetics/Epigenetics, Saarland University, 66123 Saarbrücken, Germany.

Abstract

Bisulfite sequencing is a widely used method for measuring DNA methylation in eukaryotic genomes. The assay provides single-base pair resolution and, given sufficient sequencing depth, its quantitative accuracy is excellent. High-throughput sequencing of bisulfite-converted DNA can be applied either genome wide or targeted to a defined set of genomic loci (e.g. using locus-specific PCR primers or DNA capture probes). Here, we describe BiQ Analyzer HT (http://biq-analyzer-ht.bioinf.mpi-inf.mpg.de/), a user-friendly software tool that supports locus-specific analysis and visualization of high-throughput bisulfite sequencing data. The software facilitates the shift from time-consuming clonal bisulfite sequencing to the more quantitative and cost-efficient use of high-throughput sequencing for studying locus-specific DNA methylation patterns. In addition, it is useful for locus-specific visualization of genome-wide bisulfite sequencing data.

PMID:: 21565797; [PubMed - indexed for MEDLINE]
PMCID:: PMC3125748

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