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Mol Ecol Resour. 2009 Jul;9(4):1162-4. doi: 10.1111/j.1755-0998.2009.02595.x. Epub 2009 Feb 26.

Microsatellite markers for the roman, Chrysoblephus laticeps (Teleostei: Sparidae), an overexploited seabream from South Africa.

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  • 1Molecular Ecology Laboratory, Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia. peter.teske@bio.mg.edu.au

Abstract

Eleven polymorphic microsatellite loci were developed from an enrichment library of the roman, Chrysoblephus laticeps, and characterized for 40 individuals collected in Africa's largest and oldest Marine Protected Area (MPA), the Tsitsikamma National Park. The number of alleles per locus ranged from three to 19, and heterozygosities ranged from 0.20 to 0.85. A significant departure from Hardy-Weinberg equilibrium was detected for one locus, and linkage disequilibrium was identified among three pairs of loci. The markers will be useful to detect whether populations resident in MPAs along the South African coast are genetically connected, and whether there is spillover of recruits into adjacent exploited areas.

© 2009 Blackwell Publishing Ltd.

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