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Mol Ecol Resour. 2009 May;9(3):1071-3. doi: 10.1111/j.1755-0998.2009.02579.x. Epub 2009 Mar 2.

Isolation and characterization of 16 microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae).

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  • 1Department of Biological Sciences, University of Alberta, CW 405 Biological Sciences Building, Edmonton, AB, Canada T6G 2E9, Department of Wildland Resources, Utah State University, 5230 Old Main Hill, Logan, UT 84322-5230, USA, USDA Forest Service, Rocky Mountain Research Station, 860 North 1200 East, Logan, UT 84321, USA, Natural Resources and Environmental Studies Institute, University of Northern British Columbia, 3333 University Way, Prince George, BC, Canada V2N 4Z.

Abstract

We isolated 16 polymorphic microsatellite loci in the mountain pine beetle (Dendroctonus ponderosae Hopkins) and developed conditions for amplifying these markers in four multiplex reactions. Three to 14 alleles were detected per locus across two sampled populations. Observed and expected heterozygosities ranged from 0.000 to 0.902 and from 0.100 to 0.830, respectively. Three loci deviated from Hardy-Weinberg equilibrium in one sampled population. One of these loci may be sex linked. These markers will be useful in the study of population structure in this important pest species.

© 2009 Blackwell Publishing Ltd.

PMID:
21564842
[PubMed]
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