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Mol Ecol Resour. 2009 Mar;9(2):622-4. doi: 10.1111/j.1755-0998.2008.02478.x. Epub 2009 Jan 31.

Eight novel tetranucleotide and five cross-species dinucleotide microsatellite loci for the ornate chorus frog (Pseudacris ornata).

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  • 1Department of Biology, University of Central Florida, 4000 Central Florida Blvd., Orlando, Florida 32816, USA.

Abstract

We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog (Pseudacris ornata). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymorphic. In our sample of 26 frogs from a natural population, polymorphism ranged from 1 to 22 alleles per locus with expected heterozygosities ranging from 0 to 0.958. These loci enable high-resolution studies of P. ornata. Moreover, cross-species amplification success suggests they will also be useful for other chorus frog species.

© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd.

PMID:
21564710
[PubMed]

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