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PLoS One. 2011 Apr 29;6(4):e19500. doi: 10.1371/journal.pone.0019500.

A viable hypomorphic allele of the essential IMP3 gene reveals novel protein functions in Saccharomyces cerevisiae.

Author information

  • 1IGM, CNRS, UMR 8621, Orsay, France.

Erratum in

  • PLoS One. 2011;6(5). doi:10.1371/annotation/18c67950-0fcc-4ba8-aa59-5d6a3e060c68.

Abstract

In Saccharomyces cerevisiae, the essential IMP3 gene encodes a component of the SSU processome, a large ribonucleoprotein complex required for processing of small ribosomal subunit RNA precursors. Mutation of the IMP3 termination codon to a sense codon resulted in a viable mutant allele producing a C-terminal elongated form of the Imp3 protein. A strain expressing the mutant allele displayed ribosome biogenesis defects equivalent to IMP3 depletion. This hypomorphic allele represented a unique opportunity to investigate and better understand the Imp3p functions. We demonstrated that the +1 frameshifting was increased in the mutant strain. Further characterizations revealed involvement of the Imp3 protein in DNA repair and telomere length control, pointing to a functional relationship between both pathways and ribosome biogenesis.

PMID:
21559332
[PubMed - indexed for MEDLINE]
PMCID:
PMC3084874
Free PMC Article

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