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Biomed Opt Express. 2011 Apr 22;2(5):1296-306. doi: 10.1364/BOE.2.001296.

Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system.

Abstract

We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system for hyperspectral imaging in thick tissue. We use a strategy where the Raman lines are excited sequentially, circumventing the need for a spectrometer. This fibre laser system, consisting of a pump laser synchronized with a rapidly tunable programmable laser (PL), can access Raman lines over a significant fraction of the high wavenumber region (2700-2950 cm(-1)) at rates of up to 10,000 spectral points per second. To demonstrate its capabilities, we have acquired WS-CARS spectra of several samples as well as images and hyperspectral images (HSI) of thick tissue both in forward and epi-detection. This instrument should be especially useful in providing local biochemical information with surrounding context supplied by imaging.

KEYWORDS:

(170.3880) Medical and biological imaging; (180.4315) Nonlinear microscopy; (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering

PMID:
21559141
[PubMed]
PMCID:
PMC3087586
Free PMC Article

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