Virology. 1990 Mar;175(1):149-60.

The v-rel oncogene product is complexed with cellular proteins including its proto-oncogene product and heat shock protein 70.

Source

Department of Microbiology, University of Texas, Austin 78712.

Abstract

The oncogene product, pp59v-rel, of avian reticuloendotheliosis virus (REV-T) is complexed in the cytosol of REV-T transformed lymphoid cells with cellular proteins. Monoclonal antibodies and antisera directed against different regions of pp59v-rel coimmunoprecipitate five cellular proteins (p124, p115, p75, p70, and p40) in addition to pp59v-rel. Cellular proteins with the same apparent molecular mass also copurify with pp59v-rel during sequential Sephacryl S200 and immunoaffinity chromatography. Antisera directed against the most abundant cellular protein in the complex, pp40, coimmunoprecipitate pp59v-rel and several cellular proteins with the same apparent molecular mass. The 75-kDa protein in the pp59v-rel complex is the product of c-rel proto-oncogene and is weakly phosphorylated. In MSB-1 cells this protein is not detectably phosphorylated or associated with cellular proteins. The 70-kDa protein in the pp59v-rel containing cytosolic complex is the constitutive form of avian heat shock protein 70 (HSC70). The p70 protein coimmunoprecipitates and copurifies with pp59v-rel using antisera directed against pp59v-rel and coimmunoprecipitates with antisera specific for pp40. The p70 isolated from immune complexes containing pp59v-rel shares V8 protease fragments with HSC70.

PMID:: 2155506; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

Avian reticuloendotheliosis virus-transformed lymphoid cells contain multiple pp59v-rel complexes. [J Virol. 1990]
Avian reticuloendotheliosis virus-transformed lymphoid cells contain multiple pp59v-rel complexes.
Davis N, Bargmann W, Lim MY, Bose H Jr. J Virol. 1990 Feb; 64(2):584-91.
The v-rel oncogene product is complexed to a 40-kDa phosphoprotein in transformed lymphoid cells. [Proc Natl Acad Sci U S A. 1988]
The v-rel oncogene product is complexed to a 40-kDa phosphoprotein in transformed lymphoid cells.
Tung HY, Bargmann WJ, Lim MY, Bose HR Jr. Proc Natl Acad Sci U S A. 1988 Apr; 85(8):2479-83.
Alterations within pp59v-rel-containing protein complexes following the stimulation of REV-T-transformed lymphoid cells with zinc. [Virology. 1992]
Alterations within pp59v-rel-containing protein complexes following the stimulation of REV-T-transformed lymphoid cells with zinc.
Storms RW, Bose HR Jr. Virology. 1992 Jun; 188(2):765-77.
Review Transformation of avian lymphoid cells by reticuloendotheliosis virus. [Mutat Res. 1988]
Review Transformation of avian lymphoid cells by reticuloendotheliosis virus.
Moore BE, Bose HR Jr. Mutat Res. 1988 Jan; 195(1):79-90.
Review The Rel family: models for transcriptional regulation and oncogenic transformation. [Biochim Biophys Acta. 1992]
Review The Rel family: models for transcriptional regulation and oncogenic transformation.
Bose HR Jr. Biochim Biophys Acta. 1992 Sep 14; 1114(1):1-17.

See reviews... See all...

Cited by 14 PubMed Central articles

Mutational analysis of the v-Rel dimerization interface reveals a critical role for v-Rel homodimers in transformation. [J Virol. 2002]
Mutational analysis of the v-Rel dimerization interface reveals a critical role for v-Rel homodimers in transformation.
Liss AS, Bose HR Jr. J Virol. 2002 May; 76(10):4928-39.
AP-1 factors play an important role in transformation induced by the v-rel oncogene. [Mol Cell Biol. 1998]
AP-1 factors play an important role in transformation induced by the v-rel oncogene.
Kralova J, Liss AS, Bargmann W, Bose HR Jr. Mol Cell Biol. 1998 May; 18(5):2997-3009.
Identification of a rel-related protein in the nucleus during the S phase of the cell cycle. [Mol Cell Biol. 1993]
Identification of a rel-related protein in the nucleus during the S phase of the cell cycle.
Evans RB, Gottlieb PD, Bose HR Jr. Mol Cell Biol. 1993 Oct; 13(10):6147-56.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The v-rel oncogene product is complexed with cellular proteins including its pro...
The v-rel oncogene product is complexed with cellular proteins including its proto-oncogene product and heat shock protein 70.
Virology. 1990 Mar ;175(1):149-60.

PubMed
Experience with lacosamide in a series of children with drug-resistant focal epi...
Experience with lacosamide in a series of children with drug-resistant focal epilepsy.
Pediatr Neurol. 2011 Jun ;44(6):414-9.

PubMed
Plasma homocysteine and cognitive decline in older hypertensive subjects.
Plasma homocysteine and cognitive decline in older hypertensive subjects.
Int Psychogeriatr. 2011 Dec ;23(10):1607-15. Epub 2011 May 6 .

PubMed
IP6K gene identification in plant genomes by tag searching.
IP6K gene identification in plant genomes by tag searching.
BMC Proc. 2011 May 28 ;5 Suppl 2:S1.

PubMed
Vive la différence: biogenesis and evolution of microRNAs in plants and animals.
Vive la différence: biogenesis and evolution of microRNAs in plants and animals.
Genome Biol. 2011 ;12(4):221. Epub 2011 Apr 28 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: