Mutat Res. 1990 Mar;235(2):59-63.

Restoration of radiation resistance in ataxia telangiectasia cells by the introduction of normal human chromosome 11.

Komatsu K, Kodama S, Okumura Y, Koi M, Oshimura M.

Source

Department of Radiation Biophysics, Nagasaki University School of Medicine, Japan.

Abstract

In order to identify the human chromosome which carries a mutated gene in cells from a patient with the hereditary disorder ataxia telangiectasia belonging to complementation group D (AT-D), we performed chromosome transfer experiments via microcell fusion. A single, pSV2neo-tagged chromosome, either 11 or 12, derived from normal human fibroblasts was introduced into AT-D cells by microcell fusion, and clones which were resistant to the antibiotic G418 were isolated. All 3 hybrid clones containing an additional copy number of chromosome 11 showed a restoration of the resistance of wild-type cells to killing by X-irradiation, whereas all 3 hybrid clones containing an additional copy number of chromosome 12 remained hyper-radiosensitive, like the parental AT cells. The results indicate that a defective gene of AT-D cells is also located on chromosome 11, since a genetic linkage analysis has previously suggested that a defective gene of its complementation group A is located on this chromosome.

PMID:: 2155385; [PubMed - indexed for MEDLINE]

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