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Mol Vis. 2011 Apr 27;17:1080-4.

Inhibition of proliferation of rat lens epithelial cell by overexpression of KLF6.

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  • 1Department of Ophthalmology, First Clinic College of Harbin Medical University, Harbin, China.

Abstract

PURPOSE:

Proliferation of lens epithelial cell (LEC) is main principle for posterior capsular opacity (PCO) following surgery. We investigated whether overexpression of Krüppel-like factor 6 (KLF6) can be employed to increase protein 21 (p21) and protein 27 kinase inhibition protein 1 (p27(kip1)) levels and its effect on proliferation of LEC.

METHODS:

A plasmid containing KLF6 cDNA was used to increase the level of KLF6 protein in rat lens epithelial cells (rLEC) which can lead to consequent degradation of p21 and p27(kip1). Cell proliferation was assayed by cell counts and bromodeoxyuridine (BrdU) Incorporation.

RESULTS:

western blot analysis showed increased levels of KLF6, p21, and p27(kip1) in cells transfected with pKLF6 cDNA. pKLF6 cDNA transfected cells showed less compared with control cells in vitro.

CONCLUSIONS:

pKLF6 cDNA inhibited cell proliferation and decreased cell viability of LEC by unregulation of p21 and p27(kip1).

PMID:
21552502
PMCID:
PMC3086631
[PubMed - indexed for MEDLINE]
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