Interleukin-1β and tumor necrosis factor-α co-operatively enhance a novel trophic activity of astrocytes for pontine cholinergic neurons in vitro

H Kamiguchi; K Yoshida; M Inaba; H Sasaki; M Otani; S Toya

doi:10.3233/RNN-1996-10106

Interleukin-1β and tumor necrosis factor-α co-operatively enhance a novel trophic activity of astrocytes for pontine cholinergic neurons in vitro

Restor Neurol Neurosci. 1996 Jan 1;10(1):43-7. doi: 10.3233/RNN-1996-10106.

Authors

H Kamiguchi¹, K Yoshida, M Inaba, H Sasaki, M Otani, S Toya

Affiliation

¹ Department of Neurosurgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan.

PMID: 21551852
DOI: 10.3233/RNN-1996-10106

Abstract

Insulin and insulin-like growth factors (IGFs) are the only known trophic factors for pontine cholinergic neurons. The present study revealed that astrocyte-extract pretreated with IL-1β and TNF-α significantly enhanced choline acetyltransferase (ChAT) activity of the pontine neurons in the presence of a supramaximal dose of insulin, while various trophic factors including IGFs failed to increase the ChAT activity under the same culture conditions, suggesting that IL-1β and TNF-α co-operatively enhanced the expression of a novel trophic factor for pontine cholinergic neurons in astrocytes.