Science. 1990 Feb 9;247(4943):723-6.

Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.

Sardet C, Counillon L, Franchi A, Pouysségur J.

Source

Centre de Biochimie-CNRS, Nice, France.

Abstract

The Na+/H+ antiporter, which regulates intracellular pH in virtually all cells, is one of the best examples of a mitogen- and oncogene-activated membrane target whose activity rapidly changes on stimulation. The activating mechanism is unknown. A Na+/H+ antiporter complementary DNA fragment was expressed in Escherichia coli as a beta-galactosidase fusion protein, and a specific antibody to the fusion protein was prepared. Use of this antibody revealed that the Na+/H+ antiporter is a 110-kilodalton glycoprotein that is phosphorylated in growing cells. Mitogenic activation of resting hamster fibroblasts and A431 human epidermoid cells with epidermal growth factor, thrombin, phorbol esters, or serum, stimulated phosphorylation of the Na+/H+ antiporter with a time course similar to that of the rise in intracellular pH.

PMID:: 2154036; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Medical

GLB1 Gene - Genetics Home Reference

Supplemental Content

Save items

Related citations in PubMed

Alpha-thrombin, epidermal growth factor, and okadaic acid activate the Na+/H+ exchanger, NHE-1, by phosphorylating a set of common sites. [J Biol Chem. 1991]
Alpha-thrombin, epidermal growth factor, and okadaic acid activate the Na+/H+ exchanger, NHE-1, by phosphorylating a set of common sites.
Sardet C, Fafournoux P, Pouysségur J. J Biol Chem. 1991 Oct 15; 266(29):19166-71.
Mitogen-stimulated activation of the Na+/H+ antiporter does not regulate S6 phosphorylation or protein synthesis in murine thymocytes or Swiss 3T3 fibroblasts. [J Biol Chem. 1990]
Mitogen-stimulated activation of the Na+/H+ antiporter does not regulate S6 phosphorylation or protein synthesis in murine thymocytes or Swiss 3T3 fibroblasts.
Pennington SR, Moore JP, Hesketh TR, Metcalfe JC. J Biol Chem. 1990 Feb 15; 265(5):2456-61.
Both protein kinase C and calcium mediate activation of the Na+/H+ antiporter in Chinese hamster embryo fibroblasts. [J Cell Physiol. 1987]
Both protein kinase C and calcium mediate activation of the Na+/H+ antiporter in Chinese hamster embryo fibroblasts.
Ober SS, Pardee AB. J Cell Physiol. 1987 Aug; 132(2):311-7.
Growth factors activate the bumetanide-sensitive Na+/K+/Cl-cotransport in hamster fibroblasts. [J Biol Chem. 1986]
Growth factors activate the bumetanide-sensitive Na+/K+/Cl-cotransport in hamster fibroblasts.
Paris S, Pouysségur J. J Biol Chem. 1986 May 15; 261(14):6177-83.
Review Regulation of Na+/H+ antiporter in trout red blood cells. [J Exp Biol. 1997]
Review Regulation of Na+/H+ antiporter in trout red blood cells.
Malapert M, Guizouarn H, Fievet B, Jahns R, Garcia-Romeu F, Motais R, Borgese F. J Exp Biol. 1997 Jan; 200(Pt 2):353-60.

See reviews... See all...

Cited by 46 PubMed Central articles

Nhe1 is a luminal Na+/H+ exchanger in mouse choroid plexus and is targeted to the basolateral membrane in Ncbe/Nbcn2-null mice. [Am J Physiol Cell Physiol. 2009]
Nhe1 is a luminal Na+/H+ exchanger in mouse choroid plexus and is targeted to the basolateral membrane in Ncbe/Nbcn2-null mice.
Damkier HH, Prasad V, Hübner CA, Praetorius J. Am J Physiol Cell Physiol. 2009 Jun; 296(6):C1291-300. Epub 2009 Apr 15.
Mislocalized scaffolding by the Na-H exchanger NHE1 dominantly inhibits fibronectin production and TGF-beta activation. [Mol Biol Cell. 2009]
Mislocalized scaffolding by the Na-H exchanger NHE1 dominantly inhibits fibronectin production and TGF-beta activation.
Karydis A, Jimenez-Vidal M, Denker SP, Barber DL. Mol Biol Cell. 2009 Apr; 20(8):2327-36. Epub 2009 Feb 18.
Activation of Na+/H+ and K+/H+ exchange by calyculin A in Amphiuma tridactylum red blood cells: implications for the control of volume-induced ion flux activity. [Am J Physiol Cell Physiol. 2008]
Activation of Na+/H+ and K+/H+ exchange by calyculin A in Amphiuma tridactylum red blood cells: implications for the control of volume-induced ion flux activity.
Ortiz-Acevedo A, Rigor RR, Maldonado HM, Cala PM. Am J Physiol Cell Physiol. 2008 Nov; 295(5):C1316-25. Epub 2008 Sep 17.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of ...
Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.
Science. 1990 Feb 9 ;247(4943):723-6.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Supplemental Content

Save items

Related citations in PubMed

Cited by 46 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information