Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cell Mol Life Sci. 2011 Dec;68(23):3949-61. doi: 10.1007/s00018-011-0693-7. Epub 2011 May 3.

Sox4 mediates Tbx3 transcriptional regulation of the gap junction protein Cx43.

Author information

  • 1Heart Failure Research Centre, Academic Medical Centre, University of Amsterdam, The Netherlands.

Abstract

Tbx3, a T-box transcription factor, regulates key steps in development of the heart and other organ systems. Here, we identify Sox4 as an interacting partner of Tbx3. Pull-down and nuclear retention assays verify this interaction and in situ hybridization reveals Tbx3 and Sox4 to co-localize extensively in the embryo including the atrioventricular and outflow tract cushion mesenchyme and a small area of interventricular myocardium. Tbx3, SOX4, and SOX2 ChIP data, identify a region in intron 1 of Gja1 bound by all tree proteins and subsequent ChIP experiments verify that this sequence is bound, in vivo, in the developing heart. In a luciferase reporter assay, this element displays a synergistic antagonistic response to co-transfection of Tbx3 and Sox4 and in vivo, in zebrafish, drives expression of a reporter in the heart, confirming its function as a cardiac enhancer. Mechanistically, we postulate that Sox4 is a mediator of Tbx3 transcriptional activity.

PMID:
21538160
[PubMed - indexed for MEDLINE]
PMCID:
PMC3214269
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Write to the Help Desk