Mol Imaging Biol. 2012 Apr;14(2):156-62. doi: 10.1007/s11307-011-0489-z.

In vivo photoactivation without "light": use of Cherenkov radiation to overcome the penetration limit of light.

Source

Molecular Imaging Laboratory, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, Charlestown, MA 02129, USA.

Abstract

PURPOSE:

The poor tissue penetration of visible light has been a major barrier for optical imaging, photoactivatable conversions, and photodynamic therapy for in vivo targets with depths beyond 10 mm. In this report, as a proof-of-concept, we demonstrated that a positron emission tomography (PET) radiotracer, 2-deoxy-2-[(18)F]fluoro-D-glucose ((18)FDG), could be used as an alternative light source for photoactivation.

PROCEDURES:

We utilized (18)FDG, which is a metabolic activity-based PET probe, as a source of light to photoactivate caged luciferin in a breast cancer animal model expressing luciferase.

RESULTS:

Bioluminescence produced from luciferin allowed for the real-time monitoring of Cherenkov radiation-promoted uncaging of the substrate.

CONCLUSION:

The proposed method may provide a very important option for in vivo photoactivation, in particular for activation of photosensitizers for photodynamic therapy and eventually for combining radioisotope therapy and photodynamic therapy.

PMID:: 21538154; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Diagnostic Imaging - MedlinePlus Health Information

Supplemental Content

Save items

Related citations in PubMed

In vivo ¹⁸F-FDG tumour uptake measurements in small animals using Cerenkov radiation. [Eur J Nucl Med Mol Imaging. 2011]
In vivo ¹⁸F-FDG tumour uptake measurements in small animals using Cerenkov radiation.
Boschi F, Calderan L, D'Ambrosio D, Marengo M, Fenzi A, Calandrino R, Sbarbati A, Spinelli AE. Eur J Nucl Med Mol Imaging. 2011 Jan; 38(1):120-7. Epub 2010 Sep 30.
Uptake kinetics and biodistribution of 14C-D-luciferin--a radiolabeled substrate for the firefly luciferase catalyzed bioluminescence reaction: impact on bioluminescence based reporter gene imaging. [Eur J Nucl Med Mol Imaging. 2008]
Uptake kinetics and biodistribution of 14C-D-luciferin--a radiolabeled substrate for the firefly luciferase catalyzed bioluminescence reaction: impact on bioluminescence based reporter gene imaging.
Berger F, Paulmurugan R, Bhaumik S, Gambhir SS. Eur J Nucl Med Mol Imaging. 2008 Dec; 35(12):2275-85. Epub 2008 Jul 26.
3'-deoxy-3'-[18F]fluorothymidine as a new marker for monitoring tumor response to antiproliferative therapy in vivo with positron emission tomography. [Cancer Res. 2003]
3'-deoxy-3'-[18F]fluorothymidine as a new marker for monitoring tumor response to antiproliferative therapy in vivo with positron emission tomography.
Barthel H, Cleij MC, Collingridge DR, Hutchinson OC, Osman S, He Q, Luthra SK, Brady F, Price PM, Aboagye EO. Cancer Res. 2003 Jul 1; 63(13):3791-8.
Review In vivo bioluminescence imaging. [Comp Med. 2004]
Review In vivo bioluminescence imaging.
Sato A, Klaunberg B, Tolwani R. Comp Med. 2004 Dec; 54(6):631-4.
Review [The value of 18F-fluoro-2-deoxy-D-glucose positron emission tomography (18F-FDG PET) in diagnosis of neoplastic diseases]. [Med Clin (Barc). 2005]
Review [The value of 18F-fluoro-2-deoxy-D-glucose positron emission tomography (18F-FDG PET) in diagnosis of neoplastic diseases].
Ruiz Hernández G, Romero de Avila Y Avalos C, Carreras Delgado JL. Med Clin (Barc). 2005 Feb 19; 124(6):229-36.

See reviews... See all...

Cited by 4 PubMed Central articles

In Vivo Optical Imaging of Interscapular Brown Adipose Tissue with (18)F-FDG via Cerenkov Luminescence Imaging. [PLoS One. 2013]
In Vivo Optical Imaging of Interscapular Brown Adipose Tissue with (18)F-FDG via Cerenkov Luminescence Imaging.
Zhang X, Kuo C, Moore A, Ran C. PLoS One. 2013; 8(4):e62007. Epub 2013 Apr 24.
Cerenkov imaging - a new modality for molecular imaging. [Am J Nucl Med Mol Imaging. 2012]
Cerenkov imaging - a new modality for molecular imaging.
Thorek DLj, Robertson R, Bacchus WA, Hahn J, Rothberg J, Beattie BJ, Grimm J. Am J Nucl Med Mol Imaging. 2012; 2(2):163-73. Epub 2012 Mar 28.
Quantitative modeling of Cerenkov light production efficiency from medical radionuclides. [PLoS One. 2012]
Quantitative modeling of Cerenkov light production efficiency from medical radionuclides.
Beattie BJ, Thorek DL, Schmidtlein CR, Pentlow KS, Humm JL, Hielscher AH. PLoS One. 2012; 7(2):e31402. Epub 2012 Feb 20.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

In vivo photoactivation without "light": use of Cherenkov radiation to overcome ...
In vivo photoactivation without "light": use of Cherenkov radiation to overcome the penetration limit of light.
Mol Imaging Biol. 2012 Apr ;14(2):156-62. doi: 10.1007/s11307-011-0489-z.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: