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    Clin Chem. 1979 Feb;25(2):215-7.

    New saccharogenic determination of alpha-amylase in serum and urine.

    van Leeuwen L.

    Abstract

    I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed before measurement of amylase activity is begun.

    PMID:
    215341
    [PubMed - indexed for MEDLINE]
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