Cell. 1978 Dec;15(4):1439-46.

An enzymatic activity in uninfected cells that cleaves the linkage between poliovirion RNA and the 5' terminal protein.

Abstract

The 5' terminal protein (VPg) on poliovirion RNA can be removed by cell-free extracts from a variety of uninfected cells. This soluble enzymatic activity is found in both nuclear and cytoplasmic extracts of heLa cells and is activated by Mg++. The enzyme activity cleaves the tyrosine-phosphate bond that links the protein to the RNA. In a partially purified form it has insufficient nonspecific protease or nuclease activity to account for its action. The existence of this enzyme implies that poliovirus RNA is translated in cell-free extracts in a form that lacks the 5' terminal protein. The role of this enzyme in the uninfected cell is not known.

PMID:: 215328; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Purification and properties of a HeLa cell enzyme able to remove the 5'-terminal protein from poliovirus RNA. [J Biol Chem. 1980]
Purification and properties of a HeLa cell enzyme able to remove the 5'-terminal protein from poliovirus RNA.
Ambros V, Baltimore D. J Biol Chem. 1980 Jul 25; 255(14):6739-44.
Engineered picornavirus VPg-RNA substrates: analysis of a tyrosyl-RNA phosphodiesterase activity. [PLoS One. 2011]
Engineered picornavirus VPg-RNA substrates: analysis of a tyrosyl-RNA phosphodiesterase activity.
Rozovics JM, Virgen-Slane R, Semler BL. PLoS One. 2011 Mar 7; 6(3):e16559. Epub 2011 Mar 7.
Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine. [J Biol Chem. 1978]
Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine.
Ambros V, Baltimore D. J Biol Chem. 1978 Aug 10; 253(15):5263-6.
Review The interaction of cytoplasmic RNA viruses with the nucleus. [Virus Res. 2003]
Review The interaction of cytoplasmic RNA viruses with the nucleus.
Weidman MK, Sharma R, Raychaudhuri S, Kundu P, Tsai W, Dasgupta A. Virus Res. 2003 Sep; 95(1-2):75-85.
Review Uridylylation of the genome-linked protein of poliovirus in vitro is dependent upon an endogenous RNA template. [Virus Res. 1987]
Review Uridylylation of the genome-linked protein of poliovirus in vitro is dependent upon an endogenous RNA template.
Takeda N, Yang CF, Kuhn RJ, Wimmer E. Virus Res. 1987 Sep; 8(3):193-204.

See reviews... See all...

Cited by 22 PubMed Central articles

Engineered picornavirus VPg-RNA substrates: analysis of a tyrosyl-RNA phosphodiesterase activity. [PLoS One. 2011]
Engineered picornavirus VPg-RNA substrates: analysis of a tyrosyl-RNA phosphodiesterase activity.
Rozovics JM, Virgen-Slane R, Semler BL. PLoS One. 2011 Mar 7; 6(3):e16559. Epub 2011 Mar 7.
Review Expanding knowledge of P3 proteins in the poliovirus lifecycle. [Future Microbiol. 2010]
Review Expanding knowledge of P3 proteins in the poliovirus lifecycle.
Cameron CE, Suk Oh H, Moustafa IM. Future Microbiol. 2010 Jun; 5(6):867-81.
Poliovirus 2A(Pro) increases viral mRNA and polysome stability coordinately in time with cleavage of eIF4G. [J Virol. 2008]
Poliovirus 2A(Pro) increases viral mRNA and polysome stability coordinately in time with cleavage of eIF4G.
Kempf BJ, Barton DJ. J Virol. 2008 Jun; 82(12):5847-59. Epub 2008 Apr 9.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

An enzymatic activity in uninfected cells that cleaves the linkage between polio...
An enzymatic activity in uninfected cells that cleaves the linkage between poliovirion RNA and the 5' terminal protein.
Cell. 1978 Dec ;15(4):1439-46.

PubMed
Vegetarian diets in children and adolescents.
Vegetarian diets in children and adolescents.
Paediatr Child Health. 2010 May ;15(5):303-14.

PubMed
Prognostic significance of morphological parameters and flow cytometric DNA anal...
Prognostic significance of morphological parameters and flow cytometric DNA analysis in carcinoma of the breast.
Pathol Annu. 1990 ;25 Pt 1:171-210.

PubMed
Perspectives of an interdisciplinaryg research team to engage practice: lessons ...
Perspectives of an interdisciplinaryg research team to engage practice: lessons from a knowledge exchange trainee experience.
Healthc Policy. 2010 May ;5(4):47-57.

PubMed
Spi-1 oncogene activation in Rauscher and Friend murine virus-induced acute eryt...
Spi-1 oncogene activation in Rauscher and Friend murine virus-induced acute erythroleukemias.
Leukemia. 1990 Jan ;4(1):20-3.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: