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J Bacteriol. 2011 Sep;193(18):4576-81. doi: 10.1128/JB.00325-11. Epub 2011 Apr 29.

Characteristic phenotypes associated with ptsN-null mutants in Escherichia coli K-12 are absent in strains with functional ilvG.

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  • 1Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA.

Abstract

The phosphotransferase system (PTS), encompassing EI, HPr, and assorted EII proteins, uses phosphoenolpyruvate to import and phosphorylate sugars. A paralog of EIIA of the sugar PTS system known as ptsN has been purported to regulate organic nitrogen source utilization in Escherichia coli K-12. Its known biochemical function, however, relates to potassium homeostasis. The evidence for regulation of organic nitrogen source utilization by ptsN is based primarily on the defective growth of ΔptsN mutants on amino acid nitrogen sources and other nutrient combinations. These observations were made with E. coli strains MG1655 and W3110, which carry a nonfunctional version of ilvG. There are three isozymes that effectively catalyze the first committed step of branched-chain amino acid biosynthesis, but ilvG is unique for doing so effectively across a range of potassium concentrations. Here we show that all of the nutrient utilization phenotypes attributed to ptsN are manifested selectively in strains lacking functional ilvG. We conclude that the ptsN gene product does not regulate organic nitrogen source utilization as previously proposed.

Copyright © 2011, American Society for Microbiology. All Rights Reserved.

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PMID:
21531803
[PubMed - indexed for MEDLINE]
PMCID:
PMC3165713
Free PMC Article
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