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Anal Biochem. 2011 Aug 15;415(2):175-81. doi: 10.1016/j.ab.2011.04.007. Epub 2011 Apr 15.

Gold nanoparticle-based colorimetric detection of kanamycin using a DNA aptamer.

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  • 1Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyungbuk 790-784, South Korea.

Abstract

A selective kanamycin-binding single-strand DNA (ssDNA) aptamer (TGGGGGTTGAGGCTAAGCCGA) was discovered through in vitro selection using affinity chromatography with kanamycin-immobilized sepharose beads. The selected aptamer has a high affinity for kanamycin and also for kanamycin derivatives such as kanamycin B and tobramycin. The dissociation constants (K(d) [kanamycin]=78.8 nM, K(d) [kanamycin B]=84.5 nM, and K(d) [tobramycin]=103 nM) of the new aptamer were determined by fluorescence intensity analysis using 5'-fluorescein amidite (FAM) modification. Using this aptamer, kanamycin was detected down to 25 nM by the gold nanoparticle-based colorimetric method. Because the designed colorimetric method is simple, easy, and visible to the naked eye, it has advantages that make it useful for the detection of kanamycin. Furthermore, the selected new aptamer has many potential applications as a bioprobe for the detection of kanamycin, kanamycin B, and tobramycin in pharmaceutical preparations and food products.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
21530479
[PubMed - indexed for MEDLINE]
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