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Biochem Biophys Res Commun. 2011 May 20;408(4):516-22. doi: 10.1016/j.bbrc.2011.04.040. Epub 2011 Apr 19.

Co-repressor activity of scaffold attachment factor B1 requires sumoylation.

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  • 1Breast Center and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.

Abstract

Sumoylation is an emerging modification associated with a variety of cellular processes including the regulation of transcriptional activities of nuclear receptors and their coregulators. As SUMO modifications are often associated with transcriptional repression, we examined if sumoylation was involved in modulation of the transcriptional repressive activity of scaffold attachment factor B1. Here we show that SAFB1 is modified by both the SUMO1 and SUMO2/3 family of proteins, on lysine's K231 and K294. Further, we demonstrate that SAFB1 can interact with PIAS1, a SUMO E3 ligase which mediates SAFB1 sumoylation. Additionally, SENP1 was identified as the enzyme desumoylating SAFB1. Mutation of the SAFB1 sumoylation sites lead to a loss of transcriptional repression, at least in part due to decreased interaction with HDAC3, a known transcriptional repressor and SAFB1 binding partner. In summary, the transcriptional repressor SAFB1 is modified by both SUMO1 and SUMO2/3, and this modification is necessary for its full repressive activity.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
21527249
[PubMed - indexed for MEDLINE]
PMCID:
PMC3955274
Free PMC Article

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