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Org Lett. 2011 May 20;13(10):2678-81. doi: 10.1021/ol200777d. Epub 2011 Apr 28.

Triterpene cyclases from Oryza sativa L.: cycloartenol, parkeol and achilleol B synthases.

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  • 1Department of Applied Biological Chemistry, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, Nishi-ku, Niigata, Japan.

Abstract

The gene products of AK121211, AK066327, and AK070534 from Oryza sativa encode cycloartenol, parkeol, and achilleol B synthases, respectively. Parkeol synthase is a unique enzyme that affords parkeol as a single product. Achilleol B synthase is the third seco-type triterpene cyclase identified to date, and triterpenes produced by this synthase include achilleol B (90%), tetracyclic (5.12%) and pentacyclic scaffolds (4.37%), and unidentified triterpenes (0.51%). The pathway for achilleol B biosynthesis is proposed.

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