Lack of iron-related phenotype in Sp6 intestinal knockout mice

Blood Cells Mol Dis. 2011 Jun 15;47(1):46-9. doi: 10.1016/j.bcmd.2011.03.009. Epub 2011 Apr 21.

Abstract

Based on a microarray study, which demonstrated the upregulation of Sp6 transcriptional factor in iron deficient rat duodenum, we reasoned that SP6 could regulate iron absorption by controlling the expression of iron absorption genes (Collins,2006). For that, we generated Sp6 specific intestinal knockout mice. Our data suggest a lack of transcriptional upregulation of Sp6 in mice in conditions where iron absorption is promoted (phlebotomy, iron deficiency, hpx mouse), and an absence of iron-related phenotype in Sp6 intestinal knockout model. We propose that other Sp6 orthologues may be involved in the genetic response to increase iron absorption, possibly in co-operation with hypoxia inducible factor 2 alpha (HIF-2α)-a newly discovered regulator of iron absorption.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anemia, Iron-Deficiency / genetics
  • Anemia, Iron-Deficiency / metabolism
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors / metabolism
  • Cation Transport Proteins / genetics
  • Gene Expression Regulation
  • Intestinal Mucosa / metabolism*
  • Iron / metabolism*
  • Iron Deficiencies
  • Kruppel-Like Transcription Factors / genetics*
  • Kruppel-Like Transcription Factors / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Phenotype*
  • Promoter Regions, Genetic / genetics
  • Transcriptional Activation / genetics

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Cation Transport Proteins
  • Kruppel-Like Transcription Factors
  • Sp6 protein, mouse
  • solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2
  • endothelial PAS domain-containing protein 1
  • Iron