[Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures]

Zhongguo Dang Dai Er Ke Za Zhi. 2011 Apr;13(4):285-7.
[Article in Chinese]

Abstract

Objective: To study the effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells (PBMC) of children with febrile seizures (FS).

Methods: Sixteen children with FS and 16 age- and gender-matched healthy children were enrolled. The samples of PBMC from FS children were randomized into two groups with or without TNF-α treatment (TNF-α concentration 1.0 ng/mL). PBMC were purified and cultured with a conventional method in vitro. The expression of ICAM-1 and LFA-1 in PBMC was determined by flow cytometry (FCM).

Results: ICAM-1[(20±9)% vs (14±7)%)]and LFA-1[(43±16)% vs (30±16)%]expression in PBMC in the untreated FS group was significantly higher than that in the normal control group (P<0.05). Compared with the untreated FS group, the treatment with TNF-α remarkably increased the ICAM-1 expression[(27±11)%](P<0.05). PBMC LFA-1 expression[(52±21)%]in the TNF-α-treated group was higher than that in the untreated FS group, although there were no statistical differences between the two groups.

Conclusions: TNF-α treatment may increase LFA-1 and ICAM-1 expression in PBMC of children with FS.

Publication types

  • English Abstract
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Intercellular Adhesion Molecule-1 / blood*
  • Leukocytes, Mononuclear / chemistry*
  • Leukocytes, Mononuclear / drug effects
  • Lymphocyte Function-Associated Antigen-1 / blood*
  • Male
  • Seizures, Febrile / immunology*
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Lymphocyte Function-Associated Antigen-1
  • Tumor Necrosis Factor-alpha
  • Intercellular Adhesion Molecule-1