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J Parasitol. 2011 Aug;97(4):740-2. doi: 10.1645/GE-2734.1. Epub 2011 Feb 16.

Seroprevalence of Neospora caninum and Brucella abortus in dairy cattle herds with high abortion rates.

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  • 1University Diagnostic Laboratory, University of Veterinary and Animal Sciences, Lahore 54600, Pakistan.


The protozoan Neospora caninum and the bacterium Brucella abortus are well-recognized causes of abortion in dairy cattle. Serum samples (n  =  240) from aborting (n  =  141) and at-risk (n  =  99) animals from 5 herds with high abortion rates in Punjab Province, Pakistan, were tested for antibodies to N. caninum using monoclonal antibody-based ELISA and for antibodies to B. abortus using the serum agglutination test. Antibodies to N. caninum and B. abortus were detected in 105 (43.8%) and 135 (56.3%) cattle, respectively. Prevalences of antibodies to N. caninum and B. abortus were higher in aborting cows (46.8% and 76.6%, P < 0.05) than in animals at risk (39.4% and 27.3%, P > 0.05). Sixty-six animals (27.5%) were seropositive to both N. caninum and B. abortus , and results showed no significant difference (P > 0.05) with respect to geographical district, breed, and age. This is the first report of N. caninum infection among dairy cattle herds in Pakistan.

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