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Nat Struct Mol Biol. 2011 Jun;18(6):643-9. doi: 10.1038/nsmb.2056. Epub 2011 Apr 17.

Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy.

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  • 1Laboratory of Cell Biology, The Rockefeller University, New York, New York, USA. Howard Hughes Medical Institute, Chevy Chase, Maryland, USA.

Abstract

The nuclear pore complex (NPC) perforates the nuclear envelope to facilitate selective transport between nucleus and cytoplasm. The NPC is composed of multiple copies of ∼30 different proteins, termed nucleoporins, whose arrangement within the NPC is an important unsolved puzzle in structural biology. Various alternative models for NPC architecture have been proposed but not tested experimentally in intact NPCs. We present a method using polarized fluorescence microscopy to investigate nucleoporin orientation in live yeast and mammalian cells. Our results support an arrangement of both yeast Nic96 and human Nup133-Nup107 in which their long axes are approximately parallel to the nuclear envelope plane. The method we developed can complement X-ray crystallography and electron microscopy to generate a high-resolution map of the entire NPC, and may be able to monitor nucleoporin rearrangements during nucleocytoplasmic transport and NPC assembly. This strategy can also be adapted for other macromolecular machines.

PMID:
21499242
[PubMed - indexed for MEDLINE]
PMCID:
PMC3109191
Free PMC Article

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