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Science. 2011 Apr 15;332(6027):342-6. doi: 10.1126/science.1202998.

DNA origami with complex curvatures in three-dimensional space.

Author information

  • 1The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA. dongran.han@asu.edu

Abstract

We present a strategy to design and construct self-assembling DNA nanostructures that define intricate curved surfaces in three-dimensional (3D) space using the DNA origami folding technique. Double-helical DNA is bent to follow the rounded contours of the target object, and potential strand crossovers are subsequently identified. Concentric rings of DNA are used to generate in-plane curvature, constrained to 2D by rationally designed geometries and crossover networks. Out-of-plane curvature is introduced by adjusting the particular position and pattern of crossovers between adjacent DNA double helices, whose conformation often deviates from the natural, B-form twist density. A series of DNA nanostructures with high curvature--such as 2D arrangements of concentric rings and 3D spherical shells, ellipsoidal shells, and a nanoflask--were assembled.

PMID:
21493857
[PubMed - indexed for MEDLINE]
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