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Pharmacogn Mag. 2011 Jan;7(25):40-5. doi: 10.4103/0973-1296.75900.

Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures.

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  • 1State Key Laboratory of Food Science & Technology, Nanchang University, Nanchang - 330 047, Jiangxi,, China.

Abstract

BACKGROUND:

Extraction temperature influences the total phenolic content (TPC), total flavonoid content (TFC) of medicinal plant extracts to a great extend. TPC and TFC are the principle activity constituents present in the plant. The effects of extraction temperature on TPC, TFC and free radical-scavenging capacity of Gynura divaricata leaf extracts are worth to study.

MATERIALS AND METHODS:

Folin-Ciocalteu and aluminum chloride colorimetric assay were used to determine the TPC and TFC of Gynura divaricata leaf extracts at different temperatures. The antioxidant and free radical-scavenging activity were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and phosphomolybdenum methods.

RESULTS:

TPC and TFC were significantly elevated with increasing extraction temperature (from 40°C to 100°C). However, TPC and TFC were not significantly different (P > 0.05) at the extraction temperatures 90°C and 100°C. Also, the extracts obtained at a higher temperature exhibited a significant free radical-scavenging activity compared with extraction at lower temperatures (P < 0.05). The TPCs (13.95-36.68 mg gallic acid equivalent/g dry material) were highly correlated with DPPH (R(2) = 0.9229), ABTS (R(2) = 0.9951) free radical-scavenging capacity, and total antioxidant activity (R(2) = 0.9872) evaluated by phosphomolybdenum method.

CONCLUSION:

The TPC and TFC of G. divaricata leaf was significantly influenced by the extraction temperatures, which were the main antioxidant constituents present in the G. divaricata plant.

KEYWORDS:

Antioxidant activity; Gynura divaricata; extraction temperature; total flavonoid content; total phenolic content

PMID:
21472078
[PubMed]
PMCID:
PMC3065156
Free PMC Article

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