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Nat Methods. 2011 May;8(5):409-12. doi: 10.1038/nmeth.1591. Epub 2011 Apr 3.

A more efficient method to generate integration-free human iPS cells.

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  • 1Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.


We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.

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