Nat Methods. 2011 May;8(5):409-12. doi: 10.1038/nmeth.1591. Epub 2011 Apr 3.

A more efficient method to generate integration-free human iPS cells.

Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S.

Source

Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. okita@cira.kyoto-u.ac.jp

Abstract

We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.

Comment in

Keeping things simple. [Nat Methods. 2011]
Keeping things simple.Rao M. Nat Methods. 2011 May; 8(5):389-90.

PMID:: 21460823; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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HLA Antigens

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A more efficient method to generate integration-free human iPS cells.
A more efficient method to generate integration-free human iPS cells.
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