Cell Transplant. 2011 Apr 1. [Epub ahead of print]

Feeder-free derivation of induced pluripotent stem cells from human immature dental pulp stem cells.

Beltrão-Braga PI, Pignatari GC, Maiorka PC, Oliveira NA, Lizier NF, Wenceslau CV, Miglino MA, Muotri AR, Kerkis I.

Abstract

Induced pluripotent stem cells (iPSC) can be created by forcing expression of certain genes in fibroblasts or other somatic cell types, reversing them to a pluripotent state similar to that of embryonic stem cells (ESC). Here, we used human immature dental pulp stem cells (hIDPSC) as an alternative source for creating iPSC. HIDPSC can be easily isolated from accessible tissue of young and adult patients. HIDPSC possess a fibroblast-like morphology, retaining characteristics of adult multipotent stem cells. Reprogramming of hIDPSC was fast, producing primary hIDPSC-iPSC colonies even under feeder-free conditions. hIDPSC acquired ESC-like morphology, expressed pluripotent markers, possessed stable, normal karyotypes and demonstrate the ability to differentiated in vitro and in vivo. Our data demonstrate that hIDPSC-iPSC offer an advantageous cell system for future cell therapy and basic studies, particularly as a model for pediatric developmental disorders.

PMID:: 21457612; [PubMed - as supplied by publisher]

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Feeder-free derivation of induced pluripotent stem cells from human immature dental pulp stem cells.

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