Display Settings:

Format

Send to:

Choose Destination
Int J Ayurveda Res. 2010 Oct;1(4):243-6. doi: 10.4103/0974-7788.76789.

Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristata DC), its extract, and in commercially marketed Ayurvedic dosage forms.

Author information

  • 1Dabur Research and Development Centre, Dabur India Limited, Plot No.22, Site IV, Sahibabad-201 010, Ghaziabad (U.P.), India.

Abstract

A new, simple, sensitive, selective, and precise high-performance liquid chromatography (HPLC) method for analysis of berberine in crude plant material, herbal extract, and ayurvedic dosage forms was developed and validated. The stationary phase was inert sil C(18) column The mobile phase consisting of acetonitrile (HPLC Grade) and potassium dihydrogen phosphate buffer (pH 2.5) in a gradient flow was used.The column was equilibrated with the mobile phase (flow rate 1.0 ml/min); elution was monitored at 346 nm. The linear regression analysis data for the calibration plots showed good linear relationship, with r(2)= 0.9942 in the concentration range of 16380-30420 μg/ml with respect to the peak area. The method was validated for specificity, precision, recovery, and linearity according to the International Conference on Harmonization guidelines. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of berberine.

KEYWORDS:

Berberis aristata; berberine; reverse-phase chromatography

PMID:
21455453
[PubMed]
PMCID:
PMC3059448
Free PMC Article

Images from this publication.See all images (4)Free text

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
    Loading ...
    Write to the Help Desk