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Anal Biochem. 2011 Aug 1;415(1):52-8. doi: 10.1016/j.ab.2011.03.029. Epub 2011 Mar 29.

Measuring copper and zinc superoxide dismutase from spinal cord tissue using electrospray mass spectrometry.

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  • 1Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331, USA. rhoadst@onid.orst.edu

Abstract

Metals are key cofactors for many proteins, yet quantifying the metals bound to specific proteins is a persistent challenge in vivo. We have developed a rapid and sensitive method using electrospray ionization mass spectrometry to measure Cu,Zn superoxide dismutase (SOD1) directly from the spinal cord of SOD1-overexpressing transgenic rats. Metal dyshomeostasis has been implicated in motor neuron death in amyotrophic lateral sclerosis (ALS). Using the assay, SOD1 was directly measured from 100 μg of spinal cord, allowing for anatomical quantitation of apo, metal-deficient, and holo SOD1. SOD1 was bound on a C(4) Ziptip that served as a disposable column, removing interference by physiological salts and lipids. SOD1 was eluted with 30% acetonitrile plus 100 μM formic acid to provide sufficient hydrogen ions to ionize the protein without dislodging metals. SOD1 was quantified by including bovine SOD1 as an internal standard. SOD1 could be measured in subpicomole amounts and resolved to within 2 Da of the predicted parent mass. The methods can be adapted to quantify modifications to other proteins in vivo that can be resolved by mass spectrometry.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
21453670
[PubMed - indexed for MEDLINE]
PMCID:
PMC3118564
Free PMC Article
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