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Genome Biol. 2011;12(3):R31. doi: 10.1186/gb-2011-12-3-r31. Epub 2011 Mar 31.

A vertebrate case study of the quality of assemblies derived from next-generation sequences.

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  • 1The Genome Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, MO 63108, USA.

Abstract

The unparalleled efficiency of next-generation sequencing (NGS) has prompted widespread adoption, but significant problems remain in the use of NGS data for whole genome assembly. We explore the advantages and disadvantages of chicken genome assemblies generated using a variety of sequencing and assembly methodologies. NGS assemblies are equivalent in some ways to a Sanger-based assembly yet deficient in others. Nonetheless, these assemblies are sufficient for the identification of the majority of genes and can reveal novel sequences when compared to existing assembly references.

PMID:
21453517
[PubMed - indexed for MEDLINE]
PMCID:
PMC3129681
Free PMC Article
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