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Am J Obstet Gynecol. 2011 May;204(5):450.e1-7. doi: 10.1016/j.ajog.2010.12.061. Epub 2011 Mar 27.

Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase.

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  • 1Department of Clinical Chemistry, Microbiology, and Immunology, Laboratory for Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Belgium.

Abstract

OBJECTIVE:

Sialidase and the presence of Gardnerella vaginalis have been proposed as biomarkers for bacterial vaginosis. Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase.

STUDY DESIGN:

One hundred thirty-four G vaginalis isolates were genotyped by random amplified polymorphic deoxyribonucleic acid (RAPD) and a selection of 29 isolates with amplified ribosomal deoxyribonucleic acid restriction analysis (ARDRA). A G vaginalis sialidase quantitative polymerase chain reaction was developed, and the sialidase production was assessed with the filter spot test.

RESULTS:

Three G vaginalis genotypes could be distinguished by both RAPD and ARDRA. Only 2 genotypes encoded and produced sialidase.

CONCLUSION:

Three genotypes exist among G vaginalis isolates, and there is a clear link between genotype and sialidase production. A possible link between sialidase production and (symptomatic) bacterial vaginosis and biofilm production can be hypothesized.

Copyright © 2011 Mosby, Inc. All rights reserved.

PMID:
21444061
[PubMed - indexed for MEDLINE]
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