Display Settings:

Format

Send to:

Choose Destination
Toxicol Int. 2011 Jan;18(1):62-6. doi: 10.4103/0971-6580.75866.

Lead-induced Hepatotoxicity and Evaluation of Certain Anti-stress Adaptogens in Poultry.

Author information

  • 1Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad - 500 030, Andhra Pradesh, India.

Abstract

A total of 225 day-old sexed male broiler chicks (Vencobb strain) were divided randomly into 15 groups consisting of 15 chicks in each group to study the toxicity of lead on hepatocytes. Group 1 was maintained on basal diet, group 2 on polyherbal formulation (PHF; stressroak), group 3 on shilajith, group 4 on amla and group 5 on vit E + Se. Group 6 was maintained on lead for 6 weeks and group 7 on lead for 4 weeks and subsequently on basal diet without lead for the remaining 2 weeks. Groups 8, 9, 10 and 11 were given lead along with PHF, shilajith, amla and vit E + Se, respectively, throughout 6 weeks. Groups 12, 13, 14 and 15 were given lead containing diet for the first 4 weeks and subsequently treated with PHF, shilajith, amla and vit E + Se, respectively, for the remaining 2 weeks. The activity of alanine transaminase (ALT) was significantly (P<0.05) increased in the toxic control groups at the end of 4(th) week as compared to group 1. However, following treatment, there was a significant (P<0.05) reversal in groups 12-15. The activity of Na(+)/K(+)-ATPase, Ca(2+)ATPase, Mg(2+)ATPase and CYP(450) was significantly (P<0.05) reduced in the liver of toxic control groups 6 and 7 as compared to groups1 through 5, which had the maximum activity of all the groups. Groups 8 through 15 revealed a significant (P<0.05) increase in the activity of these hepatocytic enzymes. The histological sections of the liver in lead toxic control (group 6) showed moderate focal lymphoid aggregates in liver, whereas the lesions were mild to moderate in treated groups and there were no observable lesions in plain control groups. The study revealed protective effect of PHF (stressroak), shilajith, amla and vit E + Se in lead-induced hepatocytic damage.

KEYWORDS:

ATPase; CYP450; broilers; hepatotoxicity; lead

PMID:
21430925
[PubMed]
PMCID:
PMC3052589
Free PMC Article

Images from this publication.See all images (2)Free text

Figure 1
Figure 2
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
    Loading ...
    Write to the Help Desk