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Genome Biol. 2011;12(3):R28. doi: 10.1186/gb-2011-12-3-r28. Epub 2011 Mar 23.

Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis.

Author information

  • 1School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK.

Abstract

Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.

PMID:
21429190
[PubMed - indexed for MEDLINE]
PMCID:
PMC3129678
Free PMC Article

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