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Pediatr Nephrol. 2011 Sep;26(9):1483-9. doi: 10.1007/s00467-011-1818-9. Epub 2011 Mar 15.

Two-photon in vivo imaging of cells.

Author information

  • 1The Institute of Optics, University of Rochester, Rochester, NY, USA. dan_christensen@urmc.rochester.edu

Abstract

In vivo imaging of cells gives a glimpse into the world of biology in a natural setting unparalleled by any other venue. Two-photon imaging of fluorescently labeled cells has become the standard to obtain high-resolution, dynamic images of living specimens with great specificity. This review focuses on providing the reader with a short history of, and impetus behind, two-photon imaging, its working mechanics, and emerging technologies related to biological multiphoton imaging.

PMID:
21404099
[PubMed - indexed for MEDLINE]
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