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J Neurosci Methods. 2011 May 15;198(1):99-102. doi: 10.1016/j.jneumeth.2011.03.003. Epub 2011 Mar 23.

A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells.

Author information

  • 1Department of Neurological Surgery, University of Virginia, Charlottesville, Virginia, USA. jsherman0620@gmail.com

Abstract

Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1,4-benzoquinone) for immunoflourescence staining of neurospheres.

Copyright © 2011 Elsevier B.V. All rights reserved.

PMID:
21402102
[PubMed - indexed for MEDLINE]
PMCID:
PMC3126626
Free PMC Article

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