Send to

Choose Destination
See comment in PubMed Commons below
J Neurosci Methods. 2011 May 15;198(1):99-102. doi: 10.1016/j.jneumeth.2011.03.003. Epub 2011 Mar 23.

A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells.

Author information

  • 1Department of Neurological Surgery, University of Virginia, Charlottesville, Virginia, USA.


Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1,4-benzoquinone) for immunoflourescence staining of neurospheres.

Copyright © 2011 Elsevier B.V. All rights reserved.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk