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Cell Prolif. 2011 Apr;44(2):183-91. doi: 10.1111/j.1365-2184.2011.00737.x.

Immobilization of thrombocytes on PCL nanofibres enhances chondrocyte proliferation in vitro.

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  • 1Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. jakubova@biomed.cas.cz

Abstract

OBJECTIVES:

The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention.

MATERIALS AND METHODS:

We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocyte-coated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds.

RESULTS:

Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs.

CONCLUSIONS:

These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.

© 2011 Blackwell Publishing Ltd.

PMID:
21401760
[PubMed - indexed for MEDLINE]
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