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J AOAC Int. 2011 Jan-Feb;94(1):32-42.

Recent advances in the application of high performance liquid chromatography in the analysis of polyphenols in wine and propolis.

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  • 1University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Medicinal Chemistry, A. Kovacića 1, 10000 Zagreb, Croatia.


Wine and propolis are among the richest sources of polyphenolics. Flavonoids and phenolic acids are main pharmacological components whose benefits have been shown in numerous reports. This paper presents a review of the latest applications of HPLC for determination of polyphenolics in propolis and wine. Special attention is focused on sample preparation, chromatographic conditions, and detection of polyphenolics. HPLC will undoubtedly be the most used method that is readily available for analysis, as well as for isolation of polyphenolics in complex mixtures.

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