Display Settings:


Send to:

Choose Destination
Bioanalysis. 2011 Mar;3(5):499-508. doi: 10.4155/bio.11.18.

Dual ligation hybridization assay for the specific determination of oligonucleotide therapeutics.

Author information

  • 1Charles River Preclinical & Clincal Services Montréal Inc., 22022 Transcanadienne, Senneville, QC H9X3R3, Canada. guy.tremblay@crl.com



Oligonucleotide-based therapeutics are quantified with hybridization assays in biological matrices such as plasma and tissues. Current hybridization methods do not entirely discriminate the parent compound from 5´- or 3´-N-X truncated metabolites.


A dual ligation-based hybridization assay was developed to circumvent the limitations of current assay formats. Ligation of probes at either end of the analyte is performed via a bi-enzymatic reaction consisting of polynucleotide kinase and DNA ligase. The method was validated with regard to mechanism, specificity, precision and accuracy.


The dual ligation assay is specific for the parent compound and detects the full-length product with intact 5´- and 3´-ends. The dual ligation assay can also be used to specifically determine individual metabolites in complex mixtures and is currently implemented to quantitative PCR.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Atypon
    Loading ...
    Write to the Help Desk