ACS Nano. 2011 Apr 26;5(4):2467-74. Epub 2011 Mar 9.

Site-selective binding of nanoparticles to double-stranded DNA via peptide nucleic acid "invasion".

Stadler AL, Sun D, Maye MM, van der Lelie D, Gang O.

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Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.

Abstract

We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

PMID:: 21388119; [PubMed - indexed for MEDLINE]

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Research Support, U.S. Gov't, Non-P.H.S.

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