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FEBS Lett. 2011 Apr 6;585(7):1054-60. doi: 10.1016/j.febslet.2011.03.003. Epub 2011 Mar 4.

Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor.

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  • 1Biochemical Technologies, Science and Technology Division, Corning Inc., Corning, NY 14831, United States.

Abstract

Ligand-directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label-free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist exposure and of the functional recovery of activated protease activated receptor-1 (PAR(1)). The biosensor manifests that the persistency of PAR(1) signaling is dependent on the agonists, which, in turn, affects the receptor resensitization process. This study demonstrates a new means to differentiate ligand-directed functional selectivity on trafficking of receptors.

Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

PMID:
21382370
[PubMed - indexed for MEDLINE]
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