Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cryobiology. 2011 Jun;62(3):232-41. doi: 10.1016/j.cryobiol.2011.02.006. Epub 2011 Mar 2.

On proper linearization, construction and analysis of the Boyle-van't Hoff plots and correct calculation of the osmotically inactive volume.

Author information

  • Stem Cell Center, Sanford-Burnham Medical Reserch Institute, La Jolla, CA 92037, USA. prodvincell@hotmail.com

Abstract

The Boyle-van't Hoff (BVH) law of physics has been widely used in cryobiology for calculation of the key osmotic parameters of cells and optimization of cryo-protocols. The proper use of linearization of the Boyle-vant'Hoff relationship for the osmotically inactive volume (v(b)) has been discussed in a rigorous way in (Katkov, Cryobiology, 2008, 57:142-149). Nevertheless, scientists in the field have been continuing to use inappropriate methods of linearization (and curve fitting) of the BVH data, plotting the BVH line and calculation of v(b). Here, we discuss the sources of incorrect linearization of the BVH relationship using concrete examples of recent publications, analyze the properties of the correct BVH line (which is unique for a given v(b)), provide appropriate statistical formulas for calculation of v(b) from the experimental data, and propose simplistic instructions (standard operation procedure, SOP) for proper normalization of the data, appropriate linearization and construction of the BVH plots, and correct calculation of v(b). The possible sources of non-linear behavior or poor fit of the data to the proper BVH line such as active water and/or solute transports, which can result in large discrepancy between the hyperosmotic and hypoosmotic parts of the BVH plot, are also discussed.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
21376029
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk