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Mol Biosyst. 2011 Apr;7(4):1056-9. doi: 10.1039/c0mb00338g. Epub 2011 Feb 16.

Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler.

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  • 1Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.


We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.

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